Cheltenham Town will travel away to MK Dons on Tuesday 8 March.
The previous fixture between the two clubs was postponed following an outbreak of COVID-19 cases in the Robins squad.
The March trip to Buckinghamshire will kick-off at 7.45pm at Stadium MK.
Earlier in the season when the two sides met Kyle Joseph's first half goal was cancelled out by Hiram Boateng's late leveller for MK in a game that ended 1-1.
Supporters who purchased tickets for the original fixture will be able to use the same tickets for this new date.
Those who did not buy tickets for the game will be able to when they are made available for sale.