Despite an improved second half performance and Carl Winchester's second goal in three days, the Robins lost 2-1 away at Weston-super-Mare as they too much to do after a below par first period.
Cheltenham actually had the better of the opening exchanges, with Harry Pell and Dan Holman going close.
Academy defender Jamie Kilkenny did well to clear from Weston's first dangerous attack but the hosts did manage to take the lead in the 18th minute.
Good work down the right lead to the ball being squared to Dayle Grubb and the striker finished well, making it 1-0 to the National League South side.
Weston started to dominate after the first goal and the second came as no surprise in the 27th minute. After a lovely team move, Tristen Plummer was on hand to tuck the ball into the back of the net.
For the rest of the first half, it was the home team who looked the more likely to score but it remained 2-0 at the break.
Gary Johnson made one change at the interval with Mohamed Eisa replacing Kevin Dawson, who was making his first appearance for the club. The change seemed to work with Eisa looking sharp from the outset. His attacking runs threatened the home defenders and gave the Robins some much needed momentum.
The Cheltenham goal eventually came in the 62nd minute and it was Carl Winchester, backing up his goal from Thursday at Bath, who found the net. He latched on to a good through ball and beat the keeper from an angle.
As the home side tired slightly, Cheltenham took control of the match. Jerell Sellars thought he had equalised, only to be denied by an incredibly brave block, with the Weston defender deflecting his fierce strike with his head as it looked destined for the top corner.
The Robins had a couple of more chances from corners but were unable to capitalise and the match finished 2-1.
Jon Flatt, Jamie Kilkenny (Jordon Forster, 64), Will Boyle, Dan O'Shaughnessy (Jamie Grimes, 64), Tyler Reid (Trialist, replaced by Josh Thomas, 64), Carl Winchester, (Jerell Sellars, 64), Harry Pell, Kyle Storer, Jordan Cranston (Adam Page, 86), Kevin Dawson (Mohamed Eisa, 46), Dan Holman (Nigel Atangana, 79)