Cheltenham Town's youth team play Weston-super-Mare at Whaddon Road in the first round of the FA Youth Cup on Tuesday night (7.30pm kick-off).
Weston overcame Cirencester Town in the final qualifying round to set up the tie and Robins head of youth Russell Milton has challenged his injury-hit side to avoid complacency as they bid to progress in a competition which has seen them tackle Tottenham Hotspur and Birmingham City in recent third round appearances.
"I have told my lads they will have to work hard because this is Weston's cup final and we will need to be at our best to progress," Milton said.
"I am expecting a tough game, but hopefully we'll have enough and we should be fitter."
Callum McFarlane, Liam Sheppard, Jamal Lawrence, Karnell Chambers and Dan Okojie are all sidelined with injuries, while Reece Daly (broken nose) is a doubt. James McCarthy is on the comeback trail from an ankle problem and could feature.
Goalkeeper Harry Reynolds, who has been sent to Cambridge City to gain senior experience, is too old to play in the competition, so Bojan Tetek will start between the posts.
Under-16s prospect Taylor Cordell will start at right-back, with 6ft 5in Matt Bower at centre-half. Fellow Under-16s Josh Thomas and Nathan Hughes will be among the substitutes, with Adam Page, a college Under-18s player, on the left wing.
"It's a mixed bag with so many lads injured, but it's a chance for others to impress and take their chances," Milton said.
"The boys love playing at Whaddon Road and I have told them to give a good account of themselves and try to make names for themselves because the manager (Mark Yates) and the board will be watching along with hopefully a healthy crowd."
Admission is £5 for adults and £2 for Under-16s.