League Two side Exeter City are the visitors to the Jonny-Rocks Stadium on Tuesday in the Papa John's Trophy (kick-off: 7pm).
It's Cheltenham's second southern section Group E game of this season's competition having lost on matchday one at Bristol Rovers.
Exeter drew with Chelsea under-21s in their first tie, going onto lose a penalty shootout against the Premier League youngsters, so the second of three group matches is an important matchday in the competition for both clubs.
Tickets are available to buy online for tonight's game and from the ticket office.
The Robins Retreat will again be in position in the car park as a new food offering before the game this evening.
Home fans will be able to access the Autovillage Stand, Paddock and Hazlewoods Stand this evening with Exeter fans located in blocks 7 and 8 of the Colin Farmer Stand.
Supporters are reminded that kick-off is at the earlier time of 7pm.
The game is set to provide a number of players with an opportunity to get match minutes. Kyle Vassell, Elliot Bonds and new addition Dan Crowley (pictured) are all expected to feature.
Will Boyle, Charlie Raglan and Callum Wright continue to miss out with injury problems.
Having recorded just one loss in all competitions this season, barring a penalty shootout defeat to Chelsea under-21s in the Papa John's Trophy after drawing after 90 minutes, Exeter find themselves once again pushing at the top end of the League Two table.
Former Cheltenham defender Matt Taylor's side are sixth in the fourth tier and go into tonight's fixture on the back of a 2-2 draw with Walsall on the weekend.
Taylor is expected to field a fairly strong side tonight as he has called for even more from his players in their pursuit of promotion.
On matchday one in the competition they held Chelsea under-21s to a draw and then lost on penalties with the likes of the experienced Jonathan Grounds, energetic full back Jake Caprice and Bristol City loanee Owura Edwards all featuring.
Boasting one of the best youth systems in League Two, the game may also be an opportunity for some of their academy talents to feature.
Elsewhere in the group
The other two sides in the group, Bristol Rovers and Chelsea under-21s, are due to take each other on at the Memorial Stadium on Wednesday 13 October.
Exeter and Chelsea under-21s met on matchday one and drew 1-1 after 90 minutes with Chelsea winning on penalty kicks.
Tickets bought on the day are priced at £6 for adults, £4 for seniors and £2 for under-18s and students.
You can buy online or from the ticket office ahead of the game.
With the turnstiles at the Jonny-Rocks Stadium now cashless, you must have a ticket before entering the stadium.
A 16 page copy of tonight's match programme will be on sale around the stadium priced at £2.
Watch live: The game is available to watch on iFollow. Purchase a match pass for £10 and subscribe online.
Social media: Follow @CTFCofficial on Twitter for team news and updates, CTFC on Facebook for score updates and @CTFCofficial on Instagram.
Website: Read the match report post-match and see reaction to the result from inside the camp.
Radio: Tune in to live commentary on BBC Radio Gloucestershire and on iFollow.
Highlights: Watch extended highlights on iFollow and YouTube.