Premier League 2 Division 2 side West Ham United under-21s visit the Jonny-Rocks Stadium in the Leasing.com Trophy this evening (kick-off: 7.45pm).
It's the second game of Cheltenham's Group E fixtures having lost narrowly with a young side at Exeter City on matchday one.
Newport County are still to come next month but attentions are on the young Hammers who visit tonight having won 5-4 at Newport in their first game in September.
Robins fans have been allocated the Autovillage Stand, Paddock and blocks 6, 7 and 8 of the Colin Farmer Stand for the game. West Ham supporters will be in the Hazlewoods Stand.
With Saturday's league game with Newport County postponed, a stronger side is expected to line up against the young Hammers.
The game will offer opportunities for returning pair Will Boyle and Rohan Ince to benefit from game time following their reserve team appearance last week against Oxford United under-23s.
Watch boss Michael Duff previewing the game:
The young Hammers are yet to taste defeat in Premier League 2 Division 2 this season, and including their Premier League International Cup win against Valencia, are unbeaten in 12.
They're in excellent form and will travel to the Jonny-Rocks tonight full of confidence after winning 5-4 at Newport County's Rodney Parade in their first game in the group.
Head coach Dmitri Halajko can rely on talents such as midfielder Nathan Holland, who already has a substitute appearance in the Carabao Cup for the first team, Joe Holland - who spent time on loan at Northampton last season - and Republic of Ireland under-21 international Conor Coventry.
Goalkeeper Joseph Anang has been a regular for them this season, while Anthony Scully, who has netted eight times in seven appearances so far, will be a goal threat up front. He is the son of former QPR and Notts County winger Tony Scully.
After tonight's match their next Group E fixture is at Exeter on Wednesday 13 November.
This will be the first time Cheltenham have taken on West Ham's under-21s side in any competiton. The two clubs have met previously though in cup competitions at a senior level.
The first game on 27 August 2013 came in the League Cup second round at the old Upton Park.
Goals from Ricardo Vaz Te and Ravel Morrison saw West Ham win 2-1 to progress. Matt Richards scored a penalty for Cheltenham.
The next meeting came again in the League Cup second round though this time at the LCI Rail Stadium on 23 August 2017.
West Ham won that one 2-0 with a team that included Declan Rice, Mark Noble and Andre Ayew.
Watch the highlights from that game:
You can buy tickets on the turnstiles this evening. They are priced at £6 for adults and £4 for concessions.
Cheltenham fans have been allocated the Autovillage Stand, Paddock and blocks 6, 7 and 8 of the Colin Farmer Stand.
West Ham supporters have access to the Hazlewoods Stand this evening. There is no reserved seating.
The ticket office is open ahead of the match selling tickets for upcoming home and away fixtures, including the home derby with Forest Green, and the upcoming away trip to Walsall.
Fans can also visit the club shop tonight to buy CTFC merchandise.
The match programme for tonight's game is a joint-programme with the other Leasing.com Trophy home fixture with Newport County in November.
Inside you can find out all about the night's opposition and take a look back at how the Robins got on in the first game in the group at Exeter. There's also a review of last season's campaign in the Checkatrade Trophy plus a commercial outlook too.
Keep an eye on proceedings by following @CTFCofficial on Twitter for team news, match updates, goals and in-game photos. You can also see updates on the official CTFC Facebook page too.
Watch the game online via iFollow. A match pass is available at a price of £10 for the game.
Post-match visit the club website to see reaction from manager Michael Duff and a member of the playing squad.