The draw for the second round of the Johnstone's Paint Trophy will take place on Saturday (September 4).
Cheltenham Town received a bye in the first round of this year's competition and will be paired with one of seven clubs in the South West section of the regionalised draw.
The Robins will be hoping for a better run in the competition this season having been eliminated at the first round stage last time.
The draw will take place during the Sky Sports television programme Soccer AM on Saturday morning. The programme will also feature eight Cheltenham Town supporters, who have selected to sit in the 'Luther Blissett Stand' during the show.
The regional groups for the round two draw are:
Northern Section - West
Accrington Stanley, Bury, Carlisle United, Crewe Alexandra, Macclesfield Town, Port Vale, Shrewsbury Town, Stockport County
Northern Section - East
Bradford City, Burton Albion, Chesterfield, Hartlepool United, Huddersfield Town, Peterborough United, Rotherham United, Sheffield Wednesday
Southern Section - West
Aldershot Town, Bristol Rovers, Cheltenham Town, Exeter City, Hereford United, Plymouth Argyle, Swindon Town, Torquay United
Southern Section - East
Barnet, Brentford, Charlton Athletic, Colchester United, Leyton Orient, Milton Keynes Dons, Southend United, Wycombe Wanderers